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dominant negative tcf4 dntcf4 constructs  (Addgene inc)


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    Addgene inc dominant negative tcf4 dntcf4 constructs
    Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
    Dominant Negative Tcf4 Dntcf4 Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dominant+negative+tcf4+dntcf4+constructs/pmc12593212-47-1-9?v=Addgene+inc
    Average 91 stars, based on 5 article reviews
    dominant negative tcf4 dntcf4 constructs - by Bioz Stars, 2026-07
    91/100 stars

    Images

    1) Product Images from "Targeting Wnt/β-catenin signaling enhances the efficacy of anti-CD38 immunotherapy in multiple myeloma"

    Article Title: Targeting Wnt/β-catenin signaling enhances the efficacy of anti-CD38 immunotherapy in multiple myeloma

    Journal: Neoplasia (New York, N.Y.)

    doi: 10.1016/j.neo.2025.101242

    Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either dnTCF4 or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
    Figure Legend Snippet: Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either dnTCF4 or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.

    Techniques Used: Inhibition, Western Blot, Expressing, Transduction, Plasmid Preparation, Control, CRISPR

    Inhibition of Wnt signaling upregulates CD38 expression in MM. (A) Analysis of CD38 mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (48 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; *** p ≤ 0.001 using Student’s t-test. (B) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction). A representative plot of three independent experiments is shown. (C) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction), and mean fluorescence intensity (MFI) is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Analysis of CD38 mRNA expression in the HMCLs after ICG-001 (5 µM) treatment for 24 h by qPCR. The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; ** p ≤ 0.01 using Student’s t-test. (E) Flow cytometry analysis of HMCLs cell-surface CD38 expression after ICG-001 (5 µM) treatment for 24 h.
    Figure Legend Snippet: Inhibition of Wnt signaling upregulates CD38 expression in MM. (A) Analysis of CD38 mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (48 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; *** p ≤ 0.001 using Student’s t-test. (B) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction). A representative plot of three independent experiments is shown. (C) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction), and mean fluorescence intensity (MFI) is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Analysis of CD38 mRNA expression in the HMCLs after ICG-001 (5 µM) treatment for 24 h by qPCR. The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; ** p ≤ 0.01 using Student’s t-test. (E) Flow cytometry analysis of HMCLs cell-surface CD38 expression after ICG-001 (5 µM) treatment for 24 h.

    Techniques Used: Inhibition, Expressing, Transduction, Plasmid Preparation, Flow Cytometry, Control, Fluorescence



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    dominant negative tcf4 dntcf4 constructs  (Addgene inc)
    91
    Addgene inc dominant negative tcf4 dntcf4 constructs
    Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
    Dominant Negative Tcf4 Dntcf4 Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dominant+negative+tcf4+dntcf4+constructs/pmc12593212-47-1-9?v=Addgene+inc
    Average 91 stars, based on 1 article reviews
    dominant negative tcf4 dntcf4 constructs - by Bioz Stars, 2026-07
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either dnTCF4 or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Targeting Wnt/β-catenin signaling enhances the efficacy of anti-CD38 immunotherapy in multiple myeloma

    doi: 10.1016/j.neo.2025.101242

    Figure Lengend Snippet: Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either dnTCF4 or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.

    Article Snippet: The dominant negative TCF4 (dnTCF4) constructs were obtained from Addgene (24310); The STAT3-GFP constructs were obtained from Addgene (110495) [ ].

    Techniques: Inhibition, Western Blot, Expressing, Transduction, Plasmid Preparation, Control, CRISPR

    Inhibition of Wnt signaling upregulates CD38 expression in MM. (A) Analysis of CD38 mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (48 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; *** p ≤ 0.001 using Student’s t-test. (B) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction). A representative plot of three independent experiments is shown. (C) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction), and mean fluorescence intensity (MFI) is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Analysis of CD38 mRNA expression in the HMCLs after ICG-001 (5 µM) treatment for 24 h by qPCR. The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; ** p ≤ 0.01 using Student’s t-test. (E) Flow cytometry analysis of HMCLs cell-surface CD38 expression after ICG-001 (5 µM) treatment for 24 h.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Targeting Wnt/β-catenin signaling enhances the efficacy of anti-CD38 immunotherapy in multiple myeloma

    doi: 10.1016/j.neo.2025.101242

    Figure Lengend Snippet: Inhibition of Wnt signaling upregulates CD38 expression in MM. (A) Analysis of CD38 mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (48 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; *** p ≤ 0.001 using Student’s t-test. (B) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction). A representative plot of three independent experiments is shown. (C) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction), and mean fluorescence intensity (MFI) is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Analysis of CD38 mRNA expression in the HMCLs after ICG-001 (5 µM) treatment for 24 h by qPCR. The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; ** p ≤ 0.01 using Student’s t-test. (E) Flow cytometry analysis of HMCLs cell-surface CD38 expression after ICG-001 (5 µM) treatment for 24 h.

    Article Snippet: The dominant negative TCF4 (dnTCF4) constructs were obtained from Addgene (24310); The STAT3-GFP constructs were obtained from Addgene (110495) [ ].

    Techniques: Inhibition, Expressing, Transduction, Plasmid Preparation, Flow Cytometry, Control, Fluorescence